CHINONES AS MEDIATORS FOR PHOTOMETRIC TEST

摘要

Analyte visual detection involves bringing of a sample into contact with a detection reagent, which has a nicotinamide-dependent oxidoreductase, a reducible nicotinamide coenzyme, a mediator which is a quinone, and a reducible visual indicator or a reducible visual indicator system. The analyte is oxidized by nicotinamide-dependent oxidoreductase, which reduces nicotinamide coenzyme. Electron of the reduced nicotinamide coenzyme is transferred by mediator to the visual indicator. The presence or the quantity of the analytes is determined by visual detection of indicator or indicator system. An independent claim is also included for a kit, which comprises a detection reagent and a test element, for the visual detection of an analyte in a sample, where the test element comprises an application zone for applying the sample, a reaction zone for transferring of the analyte with the detection reagent, a detection zone for determining the presence or the quantity of the analyte in the sample by a visual detection of the indicator or the indicator system, and a waste zone.