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PROCEDURE FOR DETECTING OR QUANTITATIVELY DETERMINING THE MITOCHONDRIAL DNA VARIATION 3243 AND KIT THEREFOR
PROCEDURE FOR DETECTING OR QUANTITATIVELY DETERMINING THE MITOCHONDRIAL DNA VARIATION 3243 AND KIT THEREFOR
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机译:检测或定量测定线粒体DNA变异3243和试剂盒的方法
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摘要
A method for detecting a DNA having the mitochondrial DNA 3243 mutation, using quantitative PCR using a primer having a nucleotide sequence complementary to a nucleotide sequence starting from the nucleotide number 243 in the nucleotide sequence of SEQ ID NO: 1 and having a length of 12 to 30 nucleotides, as well as, a method for detecting a DNA having the mitochondrial DNA 3243 mutation, using a nucleic acid probe of which end is labeled with a fluorescent dye, and in which fluorescence of the fluorescent dye decreases upon hybridization, wherein the nucleic acid probe has a nucleotide sequence complementary to a nucleotide sequence starting from the nucleotide number 230 in the nucleotide sequence of SEQ ID NO: 2 and having a length of 14 to 40 nucleotides, and the 3' end of the probe is labeled with the fluorescent dye.
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机译:一种使用具有与SEQ ID NO:1的核苷酸序列中的核苷酸编号243开始的核苷酸序列互补的核苷酸序列互补的核苷酸序列的引物的定量PCR,检测具有线粒体DNA 3243突变的DNA的方法。 30至30个核苷酸,以及一种检测具有线粒体DNA 3243突变的DNA的方法,该方法使用末端用荧光染料标记并且杂交后荧光染料的荧光降低的核酸探针,其中所述核酸探针具有与核苷酸序列互补的核苷酸序列,所述核苷酸序列从SEQ ID NO:2的核苷酸序列中的核苷酸编号230开始,并且具有14至40个核苷酸的长度,并且所述探针的3'端标记有荧光染料。
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