SERUM FREE MEDIA FOR STEM CELL CULTURE, TISSUE REGENERATION COMPOSITION INCLUDING THE SAME, AND METHOD OF TISSUE REGENERATION USING THE SAME

摘要

PURPOSE: the culture of serum-free stem cell and the composition comprising it are provided as enhancing the expression of the viability and growth factor of stem cell for regeneration. ;CONSTITUTION: a kind of composition for regeneration passes through the serum free medium of culture human adipocyte stem cell comprising culture solution. The serum-free culture medium contains amino acid or the like, vitamin or the like and inorganic material. L-Glutamine in amino acid or the like is 350-380 mg/litres, the l-Isoleucine in 240-270 mg/litres, in 190-220 mg/L 230-260 mg/L in Valine or L-PROLINE, the L-threonine in 190-220 mg/L. I- inositols in ascorbic acid acid-2-PO_4, the 10-30 mg/L of vitamin or its analog 10-30 mg/L, 10-20 mg/L choline chlorides or 3-10 mg/L thiamines HCl. ;The 2012 of copyright KIPO submissions