METHOD FOR SIMULTANEOUSLY ANALYZING MELATONIN AND THE PRECURSOR OF THE SAME BASED ON A GAS CHROMATOGRAPHY-MASS SPECTROMETRY CAPABLE OF IMPLEMENTING ACCURATE QUANTITATIVE ANALYSIS

摘要

PURPOSE: A method for simultaneously analyzing melatonin and the precursor of the same based on a gas chromatography-mass spectrometry is provided to derivatize the precursor of the melatonin, which is easily oxidized or decomposed, into stable materials.;CONSTITUTION: A method for simultaneously analyzing melatonin and the precursor of the same based on a gas chromatography-mass spectrometry includes the following: A mixture containing a bio specimen is reacted with ethyl chlorofomate at pH 6-8. The resultant is reacted with ethyl chlorofomate at pH 11-13. The resultant is extracted using a solvent containing diethylether and ethyl acetate in order to obtain an extract. The extract is reacted with pentafluoropropionic anhydride. The resultant is analyzed.;COPYRIGHT KIPO 2012