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A method for discriminating Chinese cabbage cultivars using molecular markers

机译:一种利用分子标记鉴别大白菜品种的方法

摘要

PURPOSE: A method for discriminating Chinese cabbage species using molecular marker is provided to use as a device for mediating infringement of species protection right of Chinese cabbage. CONSTITUTION: A marker for discriminating Chinese cabbage contains BRMS-27, BRMS-34, BRMS-43, BRMS-50, BRMS-56, KS10440, KS10621, KS10970, KS30200, KS30260, KS30270b, KS30930, KS31001, KS40270, KS40400 or KS51115 primer set. A method for discriminating species of Chinese cabbage comprises: a step of isolating genome DNA derived from Chinese cabbage; a step of amplifying DNA using a marker for discriminating species of Chinese cabbage with the genome DNA; and a step of confirming PCR product through electrophoresis and discriminating the species of Chinese cabbage.
机译:目的:提供一种利用分子标记鉴别大白菜种类的方法,作为调解大白菜种类保护权的一种手段。构成:用于鉴别大白菜的标记物包括BRMS-27,BRMS-34,BRMS-43,BRMS-50,BRMS-56,KS10440,KS10621,KS10970,KS30200,KS30260,KS30270b,KS30930,KS31001,KS40270,KS40400或KS51115底漆套。一种鉴别大白菜种类的方法,包括:分离大白菜基因组DNA的步骤;使用标记物扩增DNA的步骤,以用基因组DNA区分大白菜的种类;通过电泳确定PCR产物并鉴别大白菜种类的步骤。

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