METHOD OF DETERMINING GENETICALLY ENGINEERED MODIFIED ORGANISMS AND PHYTOGENOUS SOURCES, NON REGISTERED IN STATE LIST OF FOOD STUFF, MATERIALS AND ARTICLES, PERMITTED FOR PRODUCTION ON THE TERRITORY OF THE RUSSIAN FEDERATION OR IMPORT TO THE TERRITORY OF THE RUSSIAN FEDERATION AND TURN-ROUND, GENOME OF WHICH CONTAINS PROMOTER 35 S AND/OR TERMINATOR NOS

摘要

A method for detecting genetically modified organisms (GMOs) and sources (GMI) of plant origin, are not registered in the State register of food products, materials and products permitted for manufacture in the Russian Federation or the entry into the territory of the Russian Federation and the turnover, introduced by RF Government Decree 21 December 2000 g. №988 (hereinafter - the state register), using the polymerase chain reaction (PCR) using specific primers and fluorescently-labeled probes, complementary DNA specific to the unique transformation events GMO of plant origin (specific genetic constructions having a particular location in the genome of genetically modified organisms) recorded in a public registry, promoter 35S of cauliflower mosaic virus (cauliflower mosaic virus), the genomic DNA of the virus , terminator NOS Agrobacterium (Agrobacterium tumefasciens) and genomic DNA of Agrobacterium, characterized in that the comparison in the test sample by PCR completion: a) The number of DNA 35S promoter to the total number of recombinant DNA specific to the unique transformation events GMO vegetable origin registered in state register, whose genome comprises a 35S promoter and the genomic DNA of the cauliflower mosaic virus; ! b) a NOS terminator of DNA quantity to the total number of recombinant DNA specific to the unique transformation of plant origin GMO events recorded in the state register, the genome of which comprises a NOS terminator, and geno