CASSETTE GENETIC CONSTRUCTION, EXPRESSING THREE BIOLOGICALLY ACTIVE siRNA, EFFECTIVELY ATTACKING TRANSCRIPTS OF THE HUMAN IMMUNODEFICIENCY VIRUS AND GENE CCR5 VIA RNA- INTERFERENCE

摘要

FIELD: medicine.;SUBSTANCE: what is described in a cartridge genetic maker based on GeneClip-Ul-neo vector producing three siRNA - reproduction inhibitors of human immunodeficiency virus type 1 and human CCR5 gene. The maker contains a fragment of the length 27 bp related to a conservative region of a HIV-1 genome reverse transcriptase domain, a fragment of the length 27 bp related to the other conservative region of the HIV-1 genome reverse transcriptase domain, as well as a fragment 19 bp related to CCR5 gene mRNA. The invention can be used in medicine and scientific research.;EFFECT: invention allows producing more effective anti-HIV preparations based on interfering RNA produced in cells by means of the introduced cartridge genetic maker containing palindromes for three siRNA production.;3 dwg, 2 tbl, 2 ex