Mutant HERG channel expressing cell and use thereof

摘要

PPROBLEM TO BE SOLVED: To provide a method for three-dimensionally clarifying the interaction of a HERG channel with its inhibitor to determine the bonding position and bonding style of the inhibitor in the channel, and to provide a method for presenting the structure-modified position of the inhibitor to avoid the inhibition of the HERG channel with the inhibitor on the basis of the forecasted bonding style. PSOLUTION: A tandem dimer type protein of the -subunit of the delay rectification potassium ion channel (ISBKr/SB) of cardiac muscle cell, wherein one or both of two subunits constituting the tandem dimer are obtained by introducing a mutation to an amino acid capable of participating in the bonding of a compound inhibiting the channel, or a salt of the protein. A nucleic acid encoding the protein. An animal cell transformed with the nucleic acid. A method for forecasting the bonding style of the compound to a wild type ISBKr/SBis characterized by bringing the cell into contact with a compound inhibiting ISBKr/SBto test the inhibition of KSP+/SPchannel in the cell. PCOPYRIGHT: (C)2007,JPO&INPIT