Revelation type construction and crystallization of RNA polymerase PB1 PB2 protein of influenza virus origin

摘要

This invention makes the RNA polymerase of influenza virus origin reveal in large quantities. Crystallize the RNA polymerase of influenza virus origin. It designates that screening manner of the material which can become the active ingredient of the anti- influenza glaze is offered as purpose. This invention offers the complex which includes with the polypeptide where consists of 678-757 the amino acid array of RNA polymerase PB1 sub unit of influenza A/Puerto Rico/8/34 H1N1 die and the polypeptide where consists of 1-37 the amino acid array of RNA polymerase PB2 sub unit. The potassium dihydrogen phosphate and making crystallize under existing of the precipitant of PEG4000 and the like can do this complex. In addition, making use of the information of particular crystalline structure, it is possible to offer the screening manner of the material which can become the active ingredient of the anti- influenza glaze.