Method for Monitoring Gene Expression of Translation and Integral/Secretory Protein Synthesis by Magnetic Resonance Spectroscopy (MRS)

摘要

Disclosed is a method for monitoring change in gene expression of translation and integral/secretory protein synthesis in a sample comprising measuring the amount of Choline and/or a fatty acid present in the sample as a function of time wherein the measurement is carried out by Magnetic Resonance Spectroscopy (MRS) and correlating the amount of Choline and/or fatty acid measured as function of time to the change in gene expression of translation and integral/secretory protein synthesis in the sample. The level of Choline from organelles matches the level of Choline from transcription and integral/secretory protein synthesis. Quantification of translation and integral/secretory protein synthesis leads to quantification of genetic expression. During apoptosis the amount of Choline decreases, and the amount of fatty acids increase from distinct trafficking of Choline polar heads and fatty and tails of degraded endomembranes.