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METHOD FOR ANALYZING SECRETOME, BIOMARKER FOR LUNG CANCER METASTASIS, AND SIRNA COMPOUND FOR INHIBITING LUNG CANCER METASTASIS
METHOD FOR ANALYZING SECRETOME, BIOMARKER FOR LUNG CANCER METASTASIS, AND SIRNA COMPOUND FOR INHIBITING LUNG CANCER METASTASIS
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机译:机体分析,肺癌转移的生物标志物和抑制肺癌转移的siRNA化合物的分析方法
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摘要
A method for analyzing secretome, a biomarker for lung cancer metastasis, and a siRNA compound for inhibiting lung cancer metastasis are disclosed. The method for analyzing secretome of the present invention comprises the following steps: (A) collecting proteome secreted from a cell; (B) providing a purification gel, wherein the purification gel comprises a low-density layer, and a high-density layer, and the low-density layer is stacked on the high-density layer; (C) adding the proteome on the low-density layer, and separating the proteome through the low-density layer and the high-density layer of the purification gel; (D) collecting the separated proteome on the interface between the low-density layer and high-density layer, and tagging the separated proteome with a reagent after digestion; and (E) analyzing the separated proteome tagged with the reagent, and comparing an analysis result with a proteomic database.
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