Method of high-throughput pollen extraction, counting, and use of counted pollen for characterizing a plant

摘要

A method of quantitatively obtaining a measurement of pollen of a plant. A quantitative collection of a sample of pollen is counted. Total pollen of the plant can be calculated. One way of quantitative collection of pre-shed pollen involves blending a part of the plant with pollen in the presence of liquid (e.g. water) in a blender. The action of the blender tends to destroy the non-pollen parts of the plant and release the pollen into the liquid. Extraction of the pollen from the blender can be through a sidewall port that includes a filter or sieve having a mesh size that passes relevant pollen but blocks most non-pollen plant parts. The sample can be efficiently prepared for counting of pollen. One method of counting comprises imaging the sample with the pollen well distributed in the focal plane of the imager.