Capillary-column-based bioseparator/bioreactor with an optical/electrochemical detector for detection of microbial pathogens

摘要

The present invention is directed to satisfying the need to detect microbial contamination of food products. The described bioseparator/bioreactor coupled with an optical/electrochemical biosensor was able to specifically detect E. coli O157:H7 from 8.8×10¹ to 8.8×10⁶ CFU/ml in 2.5 hours without any enrichment. Using this invention, concentrations of S. Typhimurium ranging from 8.6×10² to 8.6×10⁶ CFU/ml in pure culture were detected in 2 hours without any enrichment. The invention may also be used for the detection of S. Seftenberg, which has the same sensitivity as S. Typhimurium. Other pathogens such as L. monocytogenes and S. Heidleberg did not interfere with the detection. The optimum inner diameter of the 25 cm long column for the detection of E. coli O157:H7 is 250 μm. The detection limit for other microbial pathogens may be controlled by changing the length of capillary columns, using higher concentration of the labeled antibodies, altering the flow rate and concentration of the substrate, and increasing the reaction temperature to 37° C.