METHOD OF CYTOGENETIC AND CYTOTOXIC EFFECTS DETECTION OF ENVIRONMENTAL FACTORS IN THYROID CELLS

摘要

The invention aims to simplify and reduce the cost of method, increase reliability, informatively and performance evaluation of various factors on thyroid gland cells of experimental animals and humans. The technical result is achieved by the cells preparation for the study carried out by fixing the organ in formalin, the cells dissociation with 50% KOH, application to dry slides, staining with azure-eosin by Romanovsky. Determine 4 cytogenetic indexes (cells with micronuclei, cells with nuclear protrusions, cells with internuclear bridges and the amount of cells with cytogenetic disorders) and 11 cytotoxic indexes (cells with atypical nuclei, dual- nuclei cells, triple- nuclei cells, cells with double nuclei, the amount of cells with three or more nuclei, mitosis, chromatin condensation, pyknosis, karyorrhexis, karyolysis, apoptosis and vacuolization of the cytoplasm) by light microscopy analysis of 1000 follicular thyrocytes (A-cells).