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A method of separating a peptidoglycan recognition protein from a hemolymph of Tenebrio molitor larvae
A method of separating a peptidoglycan recognition protein from a hemolymph of Tenebrio molitor larvae
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机译:从黄粉虫幼虫血淋巴中分离肽聚糖识别蛋白的方法
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摘要
Provided is a method of separating a peptidoglycan-recognition protein fromthe hemolymph of larvae, the method including: (a)preparing an affinity chromatography column by packing a column with a resinbound with at least one synthetic peptidoglycan; (b) applying the hemolymphof larvae to the affinity chromatography column; (c)applying a mobile phase to the affinity chromatography column to obtain a peptidoglycan-recognitionprotein-containing solution eluted from the affinity chromatography column;and (d) purifying a peptidoglycan-recognition protein from the eluted solution.
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