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A method of separating a peptidoglycan recognition protein from a hemolymph of Tenebrio molitor larvae

机译:从黄粉虫幼虫血淋巴中分离肽聚糖识别蛋白的方法

摘要

Provided is a method of separating a peptidoglycan-recognition protein fromthe hemolymph of larvae, the method including: (a)preparing an affinity chromatography column by packing a column with a resinbound with at least one synthetic peptidoglycan; (b) applying the hemolymphof larvae to the affinity chromatography column; (c)applying a mobile phase to the affinity chromatography column to obtain a peptidoglycan-recognitionprotein-containing solution eluted from the affinity chromatography column;and (d) purifying a peptidoglycan-recognition protein from the eluted solution.
机译:提供了一种从中分离肽聚糖识别蛋白的方法幼虫的血淋巴,方法包括:(a)通过用树脂填充柱制备亲和色谱柱与至少一种合成肽聚糖结合; (b)涂上淋巴液幼虫进入亲和色谱柱; (C)将流动相应用于亲和色谱柱以获得肽聚糖识别从亲和色谱柱上洗脱的含蛋白质溶液;(d)从洗脱液中纯化肽聚糖识别蛋白。

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