RECOMBINANT VIRUS-INDUCED GENE SILENCING VECTOR FROM SYMMV USEFUL FOR FUNCTIONAL ANALYSIS OF USEFUL GENES IN SOYBEAN AND USES THEREOF

摘要

The present invention relates to a recombinant virus-induced gene silencing (VIGS) vector from soybean yellow mottle mosaic virus (SYMMV), comprising a promoter and the full-length nucleic acid sequence of SYMMV, wherein a restriction enzyme site for inserting a foreign gene is included within the gene encoding the coat protein in the full-length nucleic acid sequence of SYMMV, a recombinant VIGS vector constructed by inserting phytoene desaturase (PDS) gene to the recombinant VIGS vector, a method for functional analysis of a foreign gene by using the recombinant VIGS vector, a method for production of a plant having a silenced target gene by using the recombinant VIGS vector, a plant having a silenced target gene that is produced by the method and seeds thereof, and a combination of primers useful for amplification of the recombinant VIGS vector.