EFFECTIVE PART OF GINSENG, PREPARING METHOD AND USE THEREOF

摘要

An effective part of ginseng, preparing method and use thereof, wherein the method for preparing the effective part comprises the following steps: (1) cutting ginseng into pieces, extracting with aqueous ethanol or water solution containing ethanol, concentrating the extract until relative density is 0.8-1.3 at 80℃, obtaining solution A; (2) precipitating solution A with alcohol or water, concentrating supernatant until the ratio of concentrated solution to raw medicinal material is 1:0.25-2.5, obtaining solution B; (3) adding sodium hydroxide (NaOH) into solution B until concentration of NaOH is 0.1-2 mol/L, stirring, staying for 0.5-3 hours, centrifuging or filtering, obtaining solution C, putting solution C on weak polarity or non-polar macroporous resin column, sequentially washing the column with alkaline liquid, water and 5-45% ethanol, eluting with 45-80% ethanol, collecting the eluent to obtain solution D, recycling ethanol under reduced pressure, drying, dissolving the dried substance in 5-35% ethanol, centrifuging or filtering, obtaining solution E, putting solution E on weak polarity or non-polar macroporous resin column, washing the column with 10-50% ethanol, eluting with 50-80% ethanol, collecting the eluent to obtain solution F, recycling ethanol under reduced pressure, drying to obtain effective part of ginseng. The obtained effective part of ginseng contains the following ginsenoside components (pts. wt.): protopanaxadiol saponin Rd (3-43), protopanaxadiol saponin Rg₆ (4-11), protopanaxadiol saponin F₄ (6-17), panaxatriol saponin Rk₃ (2-9), panaxatriol saponin Rh₄ (5-16), panaxatriol saponin F₂ (13-18), panaxatriol saponin Rg₃ (0.5-4), panoxadiol saponin Rk₁ (2-20), and panoxadiol saponin Rg₅ (1-30). The above mentioned effective part of ginseng has effect in preventing leucocythemia and inhibiting solid tumor cell, and no distinct toxic side effect.