ECOTOXICOLOGY ASSAY METHOD USING DEHYDROGENASE

摘要

PURPOSE: An ecotoxicology assay method using dehydrogenase is provided to improve substrate conversion rate by using iodonitrotetrazolium(INT) as substrate solution of dehydrogenase and shorten evaluation time.;CONSTITUTION: An ecotoxicology assay method using dehydrogenase comprises the following steps: mixing 2 tubes in thermo-bath after respectively placing reaction solution into centrifuge tubes which contain a control sample and a sample to process an experiment(S10); adding INT to each centrifuge tube after taking out from the thermo-bath(S20); mixing each centrifuge tube in thermo-bath which contains INT(S30); removing supernate after taking out centrifuge tubes from the thermo-bath(S50); dissolving the supernate in methanol(S60); comparing and measuring absorbance of each sample using a spectrophotometer(S70).;COPYRIGHT KIPO 2012