MICROCHIP FOR DETECTING PHENOLIC ENVIRONMENTAL HORMONE AND A DETECTION METHOD OF THE ENVIRONMENTAL HORMONES USING THE SAME

摘要

PURPOSE: A microchip for detecting phenolic environmental hormone and a detection method of the environmental hormones using the same are provided to detect the environmental hormones using electrodes having chemically modified surfaces, thereby rapidly detecting the phenolic environmental hormones at high detect rate.;CONSTITUTION: A microchip for detecting phenolic environmental hormone comprises a first concentration part which concentrate samples according to pH gradient using a field-amplified sample stacking(FASS), a concentration channel including a second concentration part which concentrates the samples according to the potential difference by using the field-amplified sample injection(FASI), and a detection channel which includes the detection part and is separated from the concentration channel having 0.05-0.2 mm interval. The detection channel comprises a carbon paste electrode reformed with gold nano-particles(AuNPs) which are stabilized with dsDNA and citric acid. The AuNPs is added to a buffer solution which is used for the concentration channel and the detection channel. The phenolic environmental hormone is 4- nonylphenol, nonylphenol, 4- octylphenol, 4- pentyl phenol or bisphenol A. The concentration channel comprises a sample injection port(101), a first sample exhausting part(102) in which the buffer solution is stored, and a part of the provided sample is exhausted, a first concentration part(103) which concentrates the samples according to pH gradient by using the field amplification sample stacking(103), a second channel(120) which connects the first sample exhausting part and the first concentration part, a first chnnel(110) which is branched from one side of the second channel, and connects the sample injection port and the second channel, a second concentration part(104) which concentrates the samples according to the potential difference by using the field amplification sample injection, a first buffer solution storage(105), and a third channel(130).;COPYRIGHT KIPO 2012