LACTATE DEHYDROGENASE-DELETED MUTANT ENTEROBACTER AEROGENES FOR IMPROVING 2,3-BUTANDIOL PRODUCTION

摘要

PURPOSE: A lactate dehydrogenase gene-deleted Enterobacter aerogenes is provided to highly prepare 2,3-butandiol and to be used as alternative energy.;CONSTITUTION: A lactate dehydrogenase gene-deleted Enterobacter aerogenes has improved 2,3-butandiol productivity. A vector for removing the gene contains flanking sequence of lactate dehydrogenase gene and FRT-kanamyucin-FRT cassette. The vector is applied to the Enterobacter aerogenes. A method for preparing the Enterobacter aerogenes comprises: a step of transforming the vector to Enterobacter aerogenes to remove the lactate dehydrogenase gene by electroporation; and a step of selecting lactate dehydrogenase gene-deleted Enterobacter aerogenes.;COPYRIGHT KIPO 2013