COMPLEX COMPOSITION OF DISULFIDE CROSSLINKED POLYETHYLENIMINE/TARGET GENE COMPLEX AND TARGET GENE FOR EFFECTIVE TRANSFECTION INTO THE CELLS

摘要

PURPOSE: A gene delivery system for transferring a target gene to cells is provided to enable stable delivery of the gene delivery system to an organism and to effectively suppress the expression of a specific disease gene. CONSTITUTION: A gene delivery system for transferring a target gene to cells is complexed by ionic interaction through mixing the target gene with polyethylene imine of Branched polyethylenimine cross-linked via disulfide bonds(ssPEI). The target gene is siRNA(small interfering RNA), an antisense molecule, an antagonist, ribozyme, an inhibitor, a peptide, or a small molecule. The target gene is siRNA which is specific to Akt gene. The target gene is fixed on a support. The support is a stent coated with hyaluronic acid or collagen on the surface.