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Detection method of Orientina tsutsugamushi using high Orientina tsutsugamushi specific primers with specific structure of a reverse complementary sequence
Detection method of Orientina tsutsugamushi using high Orientina tsutsugamushi specific primers with specific structure of a reverse complementary sequence
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机译:使用具有反向互补序列特异性结构的高东方Or虫特异性引物检测of东方虫的方法
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摘要
PURPOSE: A primer for detecting tsutsugamushi disease infection and a detection method are provided to quickly and easily determined the infection. CONSTITUTION: A primer for detecting Orientia tsutsugamushi infection comprises bases of sequence numbers 1-4. A kit for detecting Orientia tsutsugamushi infection contains the primer of sequence numbers 1-4. A method for detecting Orientia tsutsugamushi comprises: a step of isolating DNA from a test sample; a step of performing nested-PCR using the DNA and the primer of sequence numbers 1-4; and a step of analyzing the PCR product.
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