Recombinant fusion protein produced by fusing Vibrio vulnificus flagellin and pathogenic antigens and the mucosal vaccine containing the same as an active ingredient

摘要

PURPOSE: A recombinant fusion protein prepared by fusing antigen of pathogen with vaccine adjuvant and a vaccine for mucosal administration is provided to enhance PspA-specific antibody(IgG and IgA) generation. CONSTITUTION: A recombinant fusion protein is prepared by fusing protein antigen of pathogen with flagellin protein of Vibrio vulnificus. The flagellin is a FlaB of Vibrio vulnificus of sequence number 1. The antigen of pathogen is an alpha-helix domain and surface adhesion factor A(PsaA) of Streptococcus pneumoniae surface protein A(PsaA); subunit HA(hemagglutinin) and NA(neuraminidase) of influenza virus; or spike(S) protein of SARS virus. A vaccine for mucosal administration contains the recombinant fusion protein as an active ingredient.