A proteomic approach based on multiple parallel chromatography for the unambiguous identification of an antibody cognate antigen

摘要

Various chromatography using an antibody response is shown a test sample that is predicted to be included in the antigen different separation winri-present invention the antigen is not known diagnostic antibodies or self relates to a method of finding the exact antigen of the antibody, specifically, an antigen It divides the antigen source in combination of our method in parallel in a number fraction, gel electrophoresis and the antigen in the respective fractions - measuring the antibody response and an antigen-protein a mass spectrometer contained in the gel where the in fraction antibody response appears It was identified using the following, a method for identifying an antigen to find the correct protein to be detected in common in different chromatography fraction. The new method of the invention the existing antigen identification method of the false-be useful to choose exactly the antigen reactive with the antibody from the complex mixture through a combination of separation various chromatography in an efficient way up for the weak points of positive way It can be used. ;