METHOD research, in particular quantitative analysis, molecular markers, tissue MACROPHAGES absorption from the blood, which re-enter from the tissues into the bloodstream

摘要

1. A method for research, in particular quantitative analysis, molecular marker / markers absorbed by tissue macrophages from blood that are resupplied from tissues into the circulatory system, characterized by the following steps: - Treatment of whole blood agent which suppresses the coagulation and / or agglomeration of whole blood; ! - Implementation of the selection and / or enrichment and / or separation of blood macrophage or leukocyte populations containing blood macrophages from whole blood; ! - carrying out perforating or lyse macrophages or selected blood leukocyte populations containing blood macrophages, possibly after the previous optional permeabilization selected blood macrophage or leukocyte populations containing blood macrophages; ! - carrying out the qualitative and quantitative determination of markers of macrophages phagocytosed not originating from the blood, namely molecular markers derived from a tissue after a preceding punching and / or lysing blood macrophage or leukocyte populations containing blood macrophages. ! 2. A method according to claim 1, characterized in that the selection and / or enrichment and / or isolation of blood macrophages is performed by positive selection using antibodies directed against a surface marker CD16. ! 3. A method according to claim 2, characterized in that, preferably after or possibly before positive selection using antibodies directed against the surface marker CD16, positive selection is performed using antibodies directed against the surface marker CD14. ! 4. A method according to any one of claims 1-3, characterized in that