METHOD serological diagnosis of viral intestinal infections ZHULUDOCHNO CATTLE by enzyme immunoassay

摘要

Method of serological diagnosis of viral gastrointestinal infection in cattle by enzyme immunoassay, preferably rotavirus, coronavirus enteritis, viral diarrhea in cattle, comprising reacting antigens with antibodies, with antispecies antibodies labeled with horseradish peroxidase, the addition of substrate the mixture and keeping the reaction results the color intensity of the complex formed, characterized in that the reaction plates are used with pre-adsorbed antigen to them E rotavirus, coronavirus virus diarrhea and after application of test serum samples of 0.10-0.12 ml plates are incubated 1.5-2 h at 36-37 ° C, washed, then by making 0.10-0.12 Immunoassay antispecies ml total conjugate consisting of enzyme-labeled antibodies against the blood serum globulins cattle and carried out keeping the reaction results.