METHOD OF PRODUCING MONODISPERSED CARBOXYLATED POLYMER MICROSPHERES

摘要

FIELD: chemistry.;SUBSTANCE: method of producing monodispersed carboxylated polymer microspheres in form of an aqueous suspension is characterised by that a solution in a vinyl monomer of a surfactant - α,ω-bis-(10-carboxydecyl)polydimethylsiloxane, containing from 6 to 60 siloxane links and taken in amount of 0.5-1.9%, or mixture thereof with polyoxyalkylene polysiloxane of formula , where m=8-64, n=4-8, p=15-20, q=15-20, in amount of 0.9-2.6% of the weight of the monomer, is intensely stirred in bidistilled water in the atmosphere of inert gas while heating the mixture to 50-75°C and volume ratio of monomer to water in the range of 1:(2-9), with preliminary addition into the reaction mass of 0.8-1.3% (of the weight of the monomer) radical polymerisation initiators - mixture of potassium persulphate and dinitrile of azo-bis-isobutyric acid or benzoyl peroxide, to form an oil-in-water emulsion, followed by polymerisation for 2-5 hours under the action of the initiator of a drop of the monomer to polymer particles, while raising temperature by 10°C per hour until the end of the process.;EFFECT: method of producing monodispersed carboxylated polymer microspheres with concentration of carboxyl groups on the surface of the microspheres, which meets requirements for immunoreagent carriers; the suspension is stable in physiological solutions.;1 cl, 5 dwg, 11 ex