Recombinant plasmid-containing multiple copy CpG motifs and their transformants for use as DNA adjuvants in avian vaccines

摘要

A transformant of a recombinant plasmid containing multi-copy CpG motifs is disclosed. The recombinant plasmid containing an immunostimulatory polynucleotide that has a sequence of the continuous multicopy CpG motifs is transformed into a host cell to form the transformants so that the multicopy CpG motifs of the immunostimulatory polynucleotide are stable and rapid Mass production. The immunostimulatory polynucleotide, the recombinant plasmid containing the immunostimulatory polynucleotide, the transformant containing the recombinant plasmid, or any combination thereof can serve as an adjuvant for avian vaccines. Such an adjuvant can significantly reduce the effort and cost of the adjuvant, and can specifically improve the low-immunization bird vaccines and effectively solve the problem of complex chemical modification of the prior art DNA adjuvant.