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Labeling method using a peptide or a fluorescent dye for protein labeling, and fluorescent dyes
Labeling method using a peptide or a fluorescent dye for protein labeling, and fluorescent dyes
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机译:使用肽或荧光染料进行蛋白质标记的标记方法以及荧光染料
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摘要
PROBLEM TO BE SOLVED: To provide a fluorescent pigment which is easy to make labelling operations and allows a rapid and reversible labelling and a labelling method of wide application and a labelling method using the pigment.;SOLUTION: To provide a fluorescent pigment in which a ligand forming a metal complex and exhibiting a specific bonding ability to a metal-coordinating peptide and a fluorescent group are bonded through a linker. The fluorescent group has a coordinating ability to the central metal in the metal complex the ligand forms. Fluorescence extincts, as the fluorescent group coordinates with the metal. Addition of the metal-coordinating peptide breaks the coordination of the fluorescent group with the metal and fluorescence increases (recovers).;COPYRIGHT: (C)2007,JPO&INPIT
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