Three dimensional duplex film structure for cancer transfer talent evaluation method and cancer transfer talent evaluation

摘要

P To provide a method for evaluating cancer metastasis ability and in which normal cells in vitro intervene and which is approximate in vivo. PSOLUTION: This method for evaluating the cancer metastasis ability is characterized by: (Procedure 1) sequentially sticking fibroblasts to the lower side of the membrane of the first chamber having the fine pore membrane through which cancer cells can pass, sowing vascular endothelial cells ON the upper side of the membrane, and making the sheet of the fibroblasts ON the lower side of the membrane and the sheet of the vascular endothelial cells ON the upper side of the membrane and respectively; (Procedure 2) sowing cancer cells ON the sheet of the vascular endothelial cells ON the upper side of the membrane, disposing the first chamber obtained in the procedure 1 in the second chamber having a fine pore membrane through which the cancer cells disposed ON the culture plate from the upper side can not pass and culturing the cells in a culture solution in a state that the layers are overlapped at a distance; (Procedure 3) taking out the overlapped first chamber and sowing the prescribed number of cells having electric resistance ON the membrane of the second chamber having the fine pore membrane through which the left cancer cells can not pass; (Procedure 4) culturing the second chamber; and then (procedure 5) measuring the electric resistance of the second chamber with an electric resistance-measuring instrument. PCOPYRIGHT: (C)2009 and JPO& INPIT