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Reporter for RNA Polymerase II Termination
Reporter for RNA Polymerase II Termination
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机译:RNA聚合酶II终止记者
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摘要
A “tandem” reporter construct is disclosed that is capable of assaying RNA transcription termination. The ratio of expression between an upstream reporter and a downstream reporter as compared to the ratio observed for a control construct provides a measure of the relative rate of successful elongation through the intervening sequence. In one embodiment, two self-cleaving ribozymes separate the reporters from a test sequence between them.
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