A method for producing gamma-carboxylated human prothrombin isolated prothrombin produced by such method, pharmaceutical composition comprising it and use thereof as medicaments and in the manufacture of medicaments for coagulation therapy

摘要

The present invention relates to methods and tools for producing large quantities of gamma-carboxylated protein comprising: (i) culturing a cell adapted to express a protein which requires gamma-carboxylation and ³-glutamyl carboxylase in a ratio of at least 10:1, under conditions suitable for expression of both proteins, and (ii) isolating gamma-carboxylated protein