method for estimation of negative effect of cadmium on enzyme system activity of body antioxidant protection of the young cattle body

摘要

A method for estimation of negative effect of cadmium on the body of young cattle based on an analysis of the antioxidant protective system of animals by enzyme activity levels, which determines the enzymatic activity of glucose-6-phosphate content of ceruloplasmin and by comprehensive picture of the activity of enzymes of antioxidant system the extent of the negative effect of cadmium load is estimated, wherein:animals in which the activity of glutathione is within 34.6-38.4 nmol NADPH/min. to 1 mg of protein, glutathione reductase activity - within 1.49-1.64 nmol NADPH/min. to 1 mg of protein, activity of glucose-6-phosphate dehydrogenase - within 0.705-0.775 nmol NADPH/min. at 1 mg protein ceruloplasmin content - within 4.89-5.20 mmol / l, consider clinically healthy.Animals in which the activity of glutathione peroxidase - within 20.5-33.0 nmol NADPH/min. per 1 mg of protein, glutathione reductase activity - within 0.65-1.46 nmol NADPH/min. per 1 mg of protein, activity of glucose-6-phosphate dehydrogenase - within 0.52-0.699 nmol NADPH/min. per 1 mg of protein ceruloplasmin content - within 3.12-4.60 mmol/l, are considered as partially affected by cadmium, requiring correction enzymatic antioxidant protective system of the body, to them the use of natural or synthetic antioxidants and vitamins are recommended.Animals in which the activity of glutathione peroxidase is less than 20.0 nmol NADPH/min. per 1 mg of protein, glutathione reductase activity - less than 0.60 nmol NADPH/min. per 1 mg of protein, activity of glucose-6-phosphate dehydrogenase - less than 0.50 nmol NADPH/min. per 1 mg protein, ceruloplasmin content - at least 3.0 mmol/l, are considered as affected by high level of cadmium in feed with signs of irreversible metabolic disorders.