Method for determining a chemotherapeutic regimen based on ERCC1 and TS expression

摘要

A method for determining a chemotherapeutic regimen comprising 5-fluorouracil which, oxaliplatin or unacombinación thereof for treating a tumor in a patient comprising: (a) fixing a tumor sample and embedding the fixed sample in paraffin; (B) isolating mRNA from the tumor sample fixed paraffin embedded by heating the detejido sample in a solution comprising an effective concentration of a chaotropic compound to a temperature inthe range of about 75 to about 100C for a period of time aproximadamentea about 120 minutes of recovering said mRNA from said chaotropic solution; (C) subjecting the mRNA to amplification using a pair of oligonucleotide primers that hybridize condicionesrigurosas to a region of the ERCC1 gene and comprises SEQ ID NO: 1 or an identical oligonucleotide primer at least 80% thereto, and SEQ ID NO: NO 2 or an oligonucleotide primer identical to at least 80% thereto, and unpar of oligonucleotide primers hidridan under stringent conditions to a region of the TS gene conditions and comprises laSEQ ID: 3 or an oligonucleotide primer identical to at least 80% thereto, and SEQ ID NO: 4 or an identical cebadoroligonucleotídico at least 80% thereto, to obtain an amplified sample of ERCC1 and TS unamuestra amplified; (D) determining the amount of TS and ERCC1 mRNA in the amplified sample; (E) comparing the amount of TS and ERCC1 mRNA from step (d) to an amount of mRNA of a gene controlinterno; and (f) determining a chemotherapeutic regimen comprising 5-fluorouracil, oxaliplatin, or combination losmismos, based on the amount of TS mRNA and / or ERCC1 in the amplified sample and the threshold level for laexpresión the TS gene and / or ERCC1.