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A MULTICOPPER OXIDASE MUTANT, A GENE CODING THEREOF, AND A BIOFUEL-CELL USING THE SAME
A MULTICOPPER OXIDASE MUTANT, A GENE CODING THEREOF, AND A BIOFUEL-CELL USING THE SAME
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机译:多铜氧化酶突变体,编码它们的基因以及使用相同的生物燃料电池
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摘要
The present invention provides a multicopper oxidase mutant having improved resistance to imidazole compounds. A multicopper oxidase mutant, which comprises an amino acid sequence derived from the amino acid sequence shown in SEQ ID NO: 2 by substitution of either or both an amino acid residue corresponding to methionine at position 157 and an amino acid residue corresponding to proline at position 414 with a different amino acid and has activity of catalyzing a reaction generating water molecules via four-electron reduction of oxygen molecules using ABTS (2,2'-azinobis(3-ethylbenzoline-6-sulfonate)) as a substrate is provided.
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机译:本发明提供了对咪唑化合物具有改善的抗性的多铜氧化酶突变体。一种多铜氧化酶突变体,其包含由SEQ ID NO:2所示氨基酸序列衍生的氨基酸序列,该氨基酸序列是通过取代对应于位置157的蛋氨酸的氨基酸残基和对应于位置的脯氨酸的氨基酸残基中的任一个或两个提供了具有不同氨基酸的414,并且具有使用ABTS(2,2'-叠氮基双(3-乙基苯并基-6-磺酸盐))作为底物通过氧分子的四电子还原来催化产生水分子的反应的活性。
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