PRIMER SET FOR AMPLIFICATION OF MTHFR GENE, MTHFR GENE AMPLIFICATION REAGENT COMPRISING SAME, AND USE OF SAME

摘要

The present invention provides a primer set for specifically amplifying a target region in a MTHFR gene by a nucleic acid amplification method. A primer set including: a primer set (1) including a F1 primer and a R1 primer; and a primer set (2) including a F2 primer and a R2 primer is used. This allows two target regions respectively including sites at which two kinds of polymorphisms occur in the MTHFR gene to be amplified at the same time in the same reaction solution, for example.(F1) a 20- to 28-mer oligonucleotide whose 3' end is guanine (g) as the 8715th base in the base sequence of SEQ ID NO: 1(R1) an oligonucleotide complementary to an 18- to 26-mer oligonucleotide whose 5' end is cytosine(c) as the 8817th base in the base sequence of SEQ ID NO: 1(F2) a 26- to 36-mer oligonucleotide whose 3' end is guanine (g) as the 10590th base in the base sequence of SEQ ID NO: 1(R2) an oligonucleotide complementary to a 22- to 34-mer oligonucleotide whose 5' end is cytosine(c) as the 10695th base in the base sequence of SEQ ID NO: 1