A CATALYTIC TAGGING SYSTEM TO STUDY MACRO-MOLECULAR INTERACTIONS USING ENGINEERED UBIQUITIN LIGASE AND UBIQUITIN-LIKE PROTEINS TO FACILITATE SUBSTRATE IDENTIFICATION

摘要

The present invention describes an unbiased catalytic tagging system to search for target substrates. Identification of substrates for specific baits is accomplished by utilizing an orthogonal system consisting of an El activating enzyme, an E2 ubiquitin-like conjugating enzyme, and baits that are fused to the E2 ubiquitin-like conjugating enzyme. The present invention thus reveals important mutually antagonistic substrates of specific baits.