首页> 外国专利> METHOD FOR CULTURING ISLET CELLS AND METHOD FOR PREPARING CARRIER FOR ISLET CELL TRANSPLANTATION USING ATELOCOLLAGEN, AND ARTIFICIAL PANCREAS PREPARED USING SAME

METHOD FOR CULTURING ISLET CELLS AND METHOD FOR PREPARING CARRIER FOR ISLET CELL TRANSPLANTATION USING ATELOCOLLAGEN, AND ARTIFICIAL PANCREAS PREPARED USING SAME

机译:胰岛细胞的培养方法和使用胶原蛋白的胰岛细胞移植载体的制备方法以及使用该方法制备的人造胰腺

摘要

The present invention provides: a method for culturing islet cells and a method for preparing a carrier for islet cell transplantation by preparing cationic atelocollagen through the ionization of high purity atelocollagen and using the same; and an artificial pancreas prepared using the same. According to the present invention, it is possible to increase the survival rate of islet cells when culturing islet cells and/or glucose-dependent insulin secretion using cationic atelocollagen obtained through the ionization of high purity atelocollagen, or a cross-linked atelocollagen carrier, and glucose-dependent insulin secretion can be increased while simultaneously increasing the survival rate of the cultured and transplanted islet cells by providing a carrier for islet cell transplantation with excellent stability comprising cationic atelocollagen and alginates.
机译:本发明提供了一种胰岛细胞的培养方法和一种用于胰岛细胞移植的载体的方法,该方法是通过将高纯度的胶原蛋白离子化而制备阳离子化的胶原蛋白并使用它来制备的。以及使用人造胰腺制备的人造胰腺。根据本发明,当使用通过离子化高纯度胶原蛋白或交联的胶原蛋白载体获得的阳离子胶原蛋白来培养胰岛细胞和/或葡萄糖依赖性胰岛素分泌时,可以提高胰岛细胞的存活率。通过提供具有优异稳定性的用于胰岛细胞移植的载体,其中包含阳离子型端胶原和藻酸盐,可以增加葡萄糖依赖性胰岛素的分泌,同时提高培养和移植的胰岛细胞的存活率。

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