QUANTIFICATION OF POST-TRANSLATIONAL MODIFICATIONS ON HISTONE PROTEINS WITH MASS SPECTROMETRY

摘要

Provided is a highly sensitive mass spectrometry based quantitative assay for detecting histone modifications suitable for high throughput use. Also provided is a peptide reference set containing synthetic reference peptides that are direct equivalents of endogenously produced post-translationally modified histone peptides to quantify individual or a combination of histone modifications in a multiplexed manner in biological samples.