HIGH SENSITIVE ANALYZING METHOD OF ESTROGEN METABOLITES IN URINE AND BLOOD OBTAINED FROM POSTMENOPAUSAL WOMEN CAPABLE OF MEASURING THE CONCENTRATION OF ESTROGEN IN THE URINE AND THE BLOOD

摘要

PURPOSE: A high sensitive analyzing method of estrogen metabolites in urine and blood obtained from postmenopausal women is provided to verify the overall distribution of estrogen metabolism and to be used for overcoming diseases related to estrogen metabolism.;CONSTITUTION: A high sensitive analyzing method of estrogen metabolites in urine and blood obtained from postmenopausal women includes the following steps: estrogen is simultaneously extracted from urine and blood obtained from postmenopausal women and is hydrolyzed; the hydrolyzed product is alkoxy carbonylated using C1-4 alkyl chloroformate, and the resultant product is re-extracted using an organic solvent to obtain a re-extract; and the re-extract is acyl-derivated, and the concentration of estrogen is measured in the acyl-derivated product using a gas chromatography-mass spectrometry. The organic solvent is one or the mixture of normal hexane, normal pentane, butyl methyl ether, and ethyl acetate.;COPYRIGHT KIPO 2013