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Identifying Method of Marine Fishes in Southern Sea of Korea, Polynucleotide Probe, DNA Chip and Kit for Identifying The Same
Identifying Method of Marine Fishes in Southern Sea of Korea, Polynucleotide Probe, DNA Chip and Kit for Identifying The Same
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机译:韩国南海海鱼的鉴定方法,多核苷酸探针,DNA芯片和鉴定试剂盒
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摘要
The present invention relates to a method for discriminating species of 45 major fish species inhabiting the South Sea of Korea, and to polynucleotide probes, DNA chips, and kits for discriminating fish species therefor. Performing a polymerase chain reaction (PCR) to obtain a PCR product; Binding the PCR product to a probe each comprising a base sequence identical to or complementary to each DNA sequence of at least one of SEQ ID NO: 7 to SEQ ID NO: 77; And determining the species to which the fish belongs according to whether the combination is present. The present invention has the effect of analyzing the genotype for the fish inhabiting the South Sea of South Korea based on the sequence differences between species of various fish species, and can determine its species simply, quickly and accurately.
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机译:本发明涉及一种鉴别居住在韩国南海的45种主要鱼类的种类的方法,并涉及用于鉴别鱼类的多核苷酸探针,DNA芯片和试剂盒。进行聚合酶链反应(PCR)以获得PCR产物;将PCR产物与探针结合,所述探针各自包含与SEQ ID NO:7至SEQ ID NO:77中至少一个的每个DNA序列相同或互补的碱基序列;并根据组合是否存在来确定鱼类所属的物种。本发明具有基于各种鱼类的种类之间的序列差异来分析居住在韩国南海的鱼类的基因型的效果,并且可以简单,快速且准确地确定其种类。
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