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MICROSATELLITE MOLECULAR MARKER FOR SPECIFIC DISTINCTION IN CHLORELLA INTER-SPECIES AND STRAIN LEVEL

机译:微卫星分子标记在叶绿体种间和应变水平上的特殊区别

摘要

PURPOSE: A microsatellite molecular marker which is distinguishable at interspecies and intraspecies level of chlorella is provided to clearly divide chlorella within the same species. CONSTITUTION: A chlorella microsatellite specific molecular marker has a base sequence selected from sequence numbers 1-16. A primer for classifying chlorella has a base selected from sequence numbers 17-26. A composition for classifying chlorella has a primer of sequence numbers 17 and 18, 19 and 20, 21 and 22, 23 and 24, or 25 and 26. A kit for classifying chlorella contains the composition. A method for classifying chlorella comprises: a step of performing PCR using the primer for amplifying a microsatellite molecule of chlorella; and a step of classifying chlorella through fragment length polymorphism of amplified DNA.
机译:目的:提供一种在小球藻的种间和种内水平上可区分的微卫星分子标记,以清楚地区分同一物种内的小球藻。组成:小球藻微卫星特异性分子标记的碱基序列选自序列号1-16。用于对小球藻分类的引物具有选自序列号17-26的碱基。用于对小球藻分类的组合物具有序号为17和18、19和20、21和22、23和24或25和26的引物。用于对小球藻分类的试剂盒包含该组合物。对小球藻进行分类的方法包括:使用引物进行PCR以扩增小球藻的微卫星分子的步骤;以及通过扩增的DNA的片段长度多态性对小球藻进行分类的步骤。

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