METHOD OF GENETIC TEST FOR DIAGNOSIS OF MARBLING TRAIT IN KOREAN CATTLE

摘要

PURPOSE: A method for testing a gene for diagnosing Hanwoo(Korean beef) marbling is provided to diagnose an individual with AA genotype in advance. CONSTITUTION: A method for testing a gene for diagnosing Hanwoo marbling is a PCR-RFLP(Restriction Fragment Length Polymorphism) analysis method comprising a step of treating amplified an ACAD8(acyl-CoA dehydrogenase family, member 8) gene with a Drd(= Aas I, DesD I) restriction enzyme. The ACAD8 gene is amplified with a primer in sequence numbers 2 and 3. A DNA marker is detected by the base substitution at a 711th base(G to A, A to G) of the Hanwoo ACAD8 gene. Hanwoo with genetically high marbling is determined by analyzing SNP genotype(GG, GA, AA) through the method. A region containing exon 2 and exon 3 of the Hanwoo ACAD8 gene is amplified using a forward primer in sequence number 2 with 5'-ATGTAGGGTGCTCTCGAAGTC-3' and a reverse primer in sequence number 3 with 5'-CTTCAAAGATGACGGAGGTGTC-3'.