RECOMBINANT EXPRESSION VECTOR FOR PREPARING HIGH PRODUCTIVITY, HIGH IMMUNOGENICITY, AND PATHOGENICITY-FREE INFLUENZA VIRUS

摘要

PURPOSE: A recombinant expression vector for preparing high productivity, high immunogenecity, and pathogenicity-free influenza virus is provided to obtain recombinant PR8 virus with excellent immunogenecity.;CONSTITUTION: A composition for attenuating H1N1 avian influenza virus contains a polynucleotide encoding NS protein of low pathogenic avian influenza virus as an active ingredient. The NS protein is NS1 protein or NS2 protein encoded from NS gene. The NS protein contains one or more among 60th amino acid(G), 70th amino acid(F), 71th amino acid(K), 87th amino acid(T), 119th amino acid(I), 127th amino acid(T), 137th amino acid(G), 151th amino acid(T), 137th amino acid(T), 139th amino acid(G), 151th amino acid(S), 189th amino acid(N), 220th amino acid(Q), and 227th amino acid(G). NS2 protein is 31th amino acid(isoleucine), 63th amino acid(arginine), 31th amino acid(isoleucine), and 63th amino acid(arginine).;COPYRIGHT KIPO 2013;[Reference numerals] (AA) Insert into pHW2000-BsmBI