DIAGNOSING METHOD USING BRUCELLA CANIS ORIGINATED RIBOSOMAL PROTEIN L7/L12 CELL LINE EXPRESSION AND PROTEIN EXPRESSION

摘要

PURPOSE: A diagnosing method of brucellosis with high specificity and sensitivity of convenient perform due to excellent humora immunity as stimulating T lymphocyte on ribosome protein L7/L12 antigent by mass producing ribosome protein L7/L12 of Brucella canis.;CONSTITUTION: A transformed host cell comprises a gene of sequence number 1. The host cell is a yeast or Escherichia coli. A diagnosing method of Brucellosis canis comprises the following stpes. 1) A step of absorbing the ribosome protein L7/L12 antigen produced on the host cell in a immunoassay plate. 2) A step of processing sample on the absorbed plate from the step 1). 3) A step of processing the second antigen on the plate of the step 2). 4) A step of processing color former on the plate of the step 3). And 5) a step of measuring the absorbance of the plate of the step 4).;COPYRIGHT KIPO 2013;[Reference numerals] (AA) Absorbance; (BB) Negative serum; (CC) Positive serum