Probe composition for determining phylogenetic classification of animals belonging to Cetacea, phylogenetic classification DNA chip and kit, and phylogenetic classification method using the same

摘要

PURPOSE: A probe for determining classification system of animals belonging to Cetacea, DNA chips and kit including the same, and a classification determining method of animals belonging to the Cetacea using the same are provided to simply, rapidly and accurately judge genotypes of a plurality of animals belong to whale and subpopulation of the same without analyzing base sequence. CONSTITUTION: A probe for determining classification system of animals belonging to Cetacea is specifically combined with COI(Cytochrome C Oxidase subunit I) DNA region in mitochondria DNA of each species which are belong to the Cetacea. The probe comprises a probe of polynucleotide having base sequence of sequence number 3SEQ ID NO:3-14 or complementary base sequence of the polynucleotides. The length of the probe is 15 greater and 30 or less number of bases. A DNA chip for the classification system determination of the animals belonging to the Cetacea comprises probes which specifically unite with the COI gene region of the mitochondrial DNA of each animal belonging to the Cetacea. A classification system decision method of the animals belonging to the Cetacea comprises the following steps: PCR amplifying mitochondrial DNA of samples using primers for amplifying the mitochondrial DNA of each kind of animal belonging to the Cetacea; mixing the mitochondrial DNA amplified in the DNA chip; and detecting reactants which are mixed with probes of the DNA chips and determining classification system.