Recombinant yeast by overexpression of pyruvate decarboxylase and alcohol dehydrogenase for improving productivity of bioethanol and Method for producing ethanol using the same

摘要

The present invention provides pyruvate decarboxylase to a strain that inhibits the production of by-product glycerol through deletion of the glycerol production gene of Saccharomyces cerevisiae , which has been engineered to use glycerol as a fermentation source. ) And transformants with improved bioethanol production ability by overexpressing alcohol dehydrogenase and ethanol production method using the transformants, and more specifically, Saccharomyces cerevisiae ( Saccharomyces cerevisiae ) Glycerol Depletion of Glycerol-3-Phosphate Dehydrogenase 2 and FPS1 Encoding Yeast Glycerol Channel Fps1p Reduces Glycerol Production and Improves Ethanol Production Pyruvate dehydrogenation The present invention relates to a method for increasing ethanol production using a recombinant protein (pdc) encoding a carboxylic acid enzyme (pyruvate decarboxylase) and a gene (adh) encoding an alcohol dehydrogenase.