Composition for selecting green rice leafhopper-resistant variety containing Grh1 gene comprising DNA marker and method for selecting green rice leafhopper-resistant variety using DNA marker

摘要

PURPOSE: A composition containing a DNA marker for selecting Oryza sativa with green rice leaf hopper-resistant gene Grh1 is provided to efficiently distinguish green rice leaf hopper resistant and sensitive individual. CONSTITUTION: A composition for selecting Oryza sativa species with green rice leaf hopper-resistance contains a primer for detecting the presence of an oligonucleotide or a fragment thereof. The primer is one or more primer sets selected from sequence numbers 1-14. The primer set is one or more primer sets selected from sequence numbers 3-14. A method for selecting the Oryza sativa species comprises: a step of isolating genome DNA from Oryza sativa samples; a step of amplifying a target sequence using the genome DNA as a template and the primer set; and a step of detecting the amplifies product. The sample is derived from roots, leaves, stems, flowers, or buds of Oryza sativa. A kit for selecting Oryza sativa species with green rice leaf hopper-resistance contains the primer set selected from sequence numbers 1-14, restriction enzyme, and reagent.