INDUSTRIAL METHOD OF PRODUCING AND PURIFYING RECOMBINANT HUMAN INTERFERON β-1b FROM INCLUSION BODIES

摘要

FIELD: chemistry.;SUBSTANCE: disclosed is a method of producing and purifying interferon β-1b. The present method of producing and purifying interferon β-1b involves washing inclusion bodies with 0.3% solution of trifluoroacetic acid in water, followed by centrifuging, extracting protein from the washed inclusion bodies with a solution containing 1-propanol and water in ratio of 1:1, with addition of 0.3% trifluoroacetic acid, purification by reversed-phase chromatography on a C₄ sorbent, refolding in a buffer solution containing 1% sodium laurate, 50 mM Na₂HPO₄ at pH=9.0 with addition of 10 mcM copper sulphate dissolved in ammonia, extracting interferon β-1b from the aqueous solution using 75% phenol solution in water and purification by reversed-phase chromatography on a C₁₈ sorbent. Use of 0.3% trifluoroacetic acid solution for washing inclusion bodies containing β-1b is also disclosed.;EFFECT: invention enables to obtain purified interferon β-1b on industrial scales in an effective amount.;3 cl, 2 dwg, 2 tbl, 3 ex