METHOD OF DETERMINING CONTENT OF OESTROGEN AND DEXPANTHENOL IN TWO-COMPONENT MEDICAL DRUG BY HPLC

摘要

FIELD: chemistry.;SUBSTANCE: method of determining content of oestrogen and dexpanthenol in a two-component medical drug involves extraction of oestrogen and dexpanthenol from the analysed medical drug. The method also involves chromatographic extraction of oestrogen and dexpanthenol by high-performance liquid chromatography with separation on a C18 column measuring 50×2.0 mm, filled with a carrier with grain size of 2.0 mcm, or measuring 150×4.6 mm, filled with a carrier with grain size of 5 mcm, using as the mobile phases a mixture of 0.05% orthophosphoric acid solution (pH 2.4) with acetonitrile for chromatography in ratio of 90:10 to 95:5, in linear gradient conditions on a chromatograph using a UV detector. Content of oestrogen and dexpanthenol in the two-component medical drug is then determined using calculation formulae.;EFFECT: improved determination of content of oestrogen and dexpanthenol in a medical drug.;20 dwg, 3 tbl, 8 ex